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red fluorescent signal  (Sartorius AG)


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    Structured Review

    Sartorius AG red fluorescent signal
    Red Fluorescent Signal, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 15951 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/red+fluorescent+signal/Live+Cell+Analysis+Instruments/us12516099-5413-21-31
    Average 99 stars, based on 15951 article reviews
    red fluorescent signal - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Processes for generating engineered cells and compositions thereof
    Article Snippet: .. Cytolytic activity was assessed by measuring the loss of viable target cells over a period of six days, as determined by red fluorescent signal (using the IncuCyte® Live Cell Analysis System, Essen Bioscience). ..

    Article Title: Processes for generating engineered cells and compositions thereof
    Article Snippet: .. Cytolytic activity was assessed by measuring the loss of viable target cells every 4 hours over a period of 48 hours, 72 hours or 96 hours, as determined by red fluorescent signal (using the IncuCyte® Live Cell Analysis System, Essen Bioscience). ..

    Article Title: Anti-CCT5 binding molecules and chimeric antigen receptors comprising the same
    Article Snippet: Cytolytic activity was assessed by measuring the loss of viable target cells over a period of 92 hours, as determined by red fluorescent signal (using the INCUCYTE Live Cell Analysis System, Essen Bioscience). .. Cytolytic activity was assessed by measuring the loss of viable target cells over a period of 92 hours, as determined by red fluorescent signal (using the INCUCYTE® Live Cell Analysis System, Essen Bioscience). ..

    Article Title: Processes for generating engineered cells and compositions thereof
    Article Snippet: .. Cytolytic activity was assessed by measuring the loss of viable target cells over a period of 96 hours as determined by red fluorescent signal (using the IncuCyte® Live Cell Analysis System, Essen Bioscience). ..

    Cell Analysis:

    Article Title: Processes for generating engineered cells and compositions thereof
    Article Snippet: .. Cytolytic activity was assessed by measuring the loss of viable target cells over a period of six days, as determined by red fluorescent signal (using the IncuCyte® Live Cell Analysis System, Essen Bioscience). ..

    Article Title: Cells expressing a chimeric receptor from a modified CD247 locus, related polynucleotides and methods
    Article Snippet: .. The loss of NucLight Red (NLR)-labeled viable target cells was measured over 49 hours, as determined by red fluorescent signal (using the IncuCyte® Live Cell Analysis System, Essen Bioscience). ..

    Article Title: Processes for generating engineered cells and compositions thereof
    Article Snippet: .. Cytolytic activity was assessed by measuring the loss of viable target cells every 4 hours over a period of 48 hours, 72 hours or 96 hours, as determined by red fluorescent signal (using the IncuCyte® Live Cell Analysis System, Essen Bioscience). ..

    Article Title: Cells expressing a chimeric receptor from a modified CD247 locus, related polynucleotides and methods
    Article Snippet: .. The cells were incubated with labeled MM.1S (ATCC® CRL-2974TM) human B lymphoblast target cells, at an effector:target (E:T) ratio of 2:1 or 1:2, and the loss of NucLight Red (NLR)-labeled viable target cells was measured over three days, as determined by red fluorescent signal (using the IncuCyte® Live Cell Analysis System, Essen Bioscience). ..

    Article Title: Anti-CCT5 binding molecules and chimeric antigen receptors comprising the same
    Article Snippet: Cytolytic activity was assessed by measuring the loss of viable target cells over a period of 92 hours, as determined by red fluorescent signal (using the INCUCYTE Live Cell Analysis System, Essen Bioscience). .. Cytolytic activity was assessed by measuring the loss of viable target cells over a period of 92 hours, as determined by red fluorescent signal (using the INCUCYTE® Live Cell Analysis System, Essen Bioscience). ..

    Article Title: Processes for generating engineered cells and compositions thereof
    Article Snippet: .. Cytolytic activity was assessed by measuring the loss of viable target cells over a period of 96 hours as determined by red fluorescent signal (using the IncuCyte® Live Cell Analysis System, Essen Bioscience). ..

    Incubation:

    Article Title: Cells expressing a chimeric receptor from a modified CD247 locus, related polynucleotides and methods
    Article Snippet: .. The cells were incubated with labeled MM.1S (ATCC® CRL-2974TM) human B lymphoblast target cells, at an effector:target (E:T) ratio of 2:1 or 1:2, and the loss of NucLight Red (NLR)-labeled viable target cells was measured over three days, as determined by red fluorescent signal (using the IncuCyte® Live Cell Analysis System, Essen Bioscience). ..

    Labeling:

    Article Title: Cells expressing a chimeric receptor from a modified CD247 locus, related polynucleotides and methods
    Article Snippet: .. The cells were incubated with labeled MM.1S (ATCC® CRL-2974TM) human B lymphoblast target cells, at an effector:target (E:T) ratio of 2:1 or 1:2, and the loss of NucLight Red (NLR)-labeled viable target cells was measured over three days, as determined by red fluorescent signal (using the IncuCyte® Live Cell Analysis System, Essen Bioscience). ..



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    EC-specific galectin-7 overexpression inhibits disturbed flow-induced EndMT, and EC-specific galectin-7 knockdown promotes disturbed flow-induced EndMT in AngII- and PPE-induced AAA. A and E , Immunofluorescence staining and corresponding densitometric analysis of CD31 (green) and vimentin (red) in the 2 indicated groups. Nuclei were costained with DAPI (blue) (scale bars = 50 μm; n = 4/group). B and F , Immunofluorescence staining and corresponding densitometric analysis of CD31 (green) and fibronectin (red) in the 2 indicated groups. Nuclei were costained with DAPI (blue) (scale bars = 50 μm; n = 4/group). C and G , Immunofluorescence staining and corresponding densitometric analysis of CD31 (green) and vimentin (red) in the 2 indicated groups. Nuclei were costained with DAPI (blue) (scale bars = 50 μm; n = 4/group). D and H , Immunofluorescence staining and corresponding densitometric analysis of CD31 (green) and fibronectin (red) in the 2 indicated groups. Nuclei were costained with DAPI (blue) (scale bars = 50 μm; n = 4/group). I-J . Immunofluorescence staining and corresponding densitometric analysis of <t>tdTomato</t> (red) and fibronectin (green) in Cdh5-CreERT2 mouse aortas in the 2 indicated groups (scale bars = 50 μm, n = 3/group). K-L . Immunofluorescence staining and corresponding densitometric analysis of tdTomato (red) and fibronectin (green) in Cdh5-CreERT2 mouse aortas in the 2 indicated groups (scale bars = 50 μm, n = 3/group). Unpaired Student's t tests were performed for E-H, J and L. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
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    Addgene inc red-fluorescent-tagged amino-terminal domain of kar2 fused to the hdel er retention signal
    Cellular location of Ded1 relative to the ER and SRP proteins. ( A ) Ded1-DQAD-GFP was expressed in the sec62 temperature-sensitive mutant with the integrated <t>KAR2-RFP</t> plasmid and grown to an OD600 of 1.0 at 24 °C. ( B ) The same cells as in A were incubated for 15 min at the non-permissive temperature of 37 °C prior to visualization. The arrowheads indicate positions where chains of Ded1-DQAD-GFP foci co-associated with Kar2-RFP in panels B and C. ( C ) SRP14-GFP expressed from the chromosome and Ded1-DQAD-mCh expressed off the p415 plasmid were grown to an OD600 of 0.95 at 30 °C. ( D ) SRP14-GFP was overexpressed off the p413-PL plasmid and Ded1-DQAD-mCh was overexpressed off the p416-PL plasmid until an OD600 of 0.4 at 30 °C in the xpoI-T539C yeast strain. Cells in the insert were treated with 10 µg/µL (~200 nM) of leptomycin b for 1 h. The arrowheads indicate the positions where Ded1-DQAD and SRP14 colocalize within a crescent-shaped region of the nucleus.
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    Image Search Results


    EC-specific galectin-7 overexpression inhibits disturbed flow-induced EndMT, and EC-specific galectin-7 knockdown promotes disturbed flow-induced EndMT in AngII- and PPE-induced AAA. A and E , Immunofluorescence staining and corresponding densitometric analysis of CD31 (green) and vimentin (red) in the 2 indicated groups. Nuclei were costained with DAPI (blue) (scale bars = 50 μm; n = 4/group). B and F , Immunofluorescence staining and corresponding densitometric analysis of CD31 (green) and fibronectin (red) in the 2 indicated groups. Nuclei were costained with DAPI (blue) (scale bars = 50 μm; n = 4/group). C and G , Immunofluorescence staining and corresponding densitometric analysis of CD31 (green) and vimentin (red) in the 2 indicated groups. Nuclei were costained with DAPI (blue) (scale bars = 50 μm; n = 4/group). D and H , Immunofluorescence staining and corresponding densitometric analysis of CD31 (green) and fibronectin (red) in the 2 indicated groups. Nuclei were costained with DAPI (blue) (scale bars = 50 μm; n = 4/group). I-J . Immunofluorescence staining and corresponding densitometric analysis of tdTomato (red) and fibronectin (green) in Cdh5-CreERT2 mouse aortas in the 2 indicated groups (scale bars = 50 μm, n = 3/group). K-L . Immunofluorescence staining and corresponding densitometric analysis of tdTomato (red) and fibronectin (green) in Cdh5-CreERT2 mouse aortas in the 2 indicated groups (scale bars = 50 μm, n = 3/group). Unpaired Student's t tests were performed for E-H, J and L. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

    Journal: Theranostics

    Article Title: Galectin7 attenuates abdominal aortic aneurysm progression by resisting disturbed flow induced endothelial-to-mesenchymal transition

    doi: 10.7150/thno.117785

    Figure Lengend Snippet: EC-specific galectin-7 overexpression inhibits disturbed flow-induced EndMT, and EC-specific galectin-7 knockdown promotes disturbed flow-induced EndMT in AngII- and PPE-induced AAA. A and E , Immunofluorescence staining and corresponding densitometric analysis of CD31 (green) and vimentin (red) in the 2 indicated groups. Nuclei were costained with DAPI (blue) (scale bars = 50 μm; n = 4/group). B and F , Immunofluorescence staining and corresponding densitometric analysis of CD31 (green) and fibronectin (red) in the 2 indicated groups. Nuclei were costained with DAPI (blue) (scale bars = 50 μm; n = 4/group). C and G , Immunofluorescence staining and corresponding densitometric analysis of CD31 (green) and vimentin (red) in the 2 indicated groups. Nuclei were costained with DAPI (blue) (scale bars = 50 μm; n = 4/group). D and H , Immunofluorescence staining and corresponding densitometric analysis of CD31 (green) and fibronectin (red) in the 2 indicated groups. Nuclei were costained with DAPI (blue) (scale bars = 50 μm; n = 4/group). I-J . Immunofluorescence staining and corresponding densitometric analysis of tdTomato (red) and fibronectin (green) in Cdh5-CreERT2 mouse aortas in the 2 indicated groups (scale bars = 50 μm, n = 3/group). K-L . Immunofluorescence staining and corresponding densitometric analysis of tdTomato (red) and fibronectin (green) in Cdh5-CreERT2 mouse aortas in the 2 indicated groups (scale bars = 50 μm, n = 3/group). Unpaired Student's t tests were performed for E-H, J and L. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

    Article Snippet: Lineage tracing revealed that compared with that in the arterial medial layer in the control group, the tdTomato red fluorescence signal in the arterial medial layer was reduced in the galectin-7 overexpression group, indicating a decrease in EndMT.

    Techniques: Over Expression, Knockdown, Immunofluorescence, Staining

    Cellular location of Ded1 relative to the ER and SRP proteins. ( A ) Ded1-DQAD-GFP was expressed in the sec62 temperature-sensitive mutant with the integrated KAR2-RFP plasmid and grown to an OD600 of 1.0 at 24 °C. ( B ) The same cells as in A were incubated for 15 min at the non-permissive temperature of 37 °C prior to visualization. The arrowheads indicate positions where chains of Ded1-DQAD-GFP foci co-associated with Kar2-RFP in panels B and C. ( C ) SRP14-GFP expressed from the chromosome and Ded1-DQAD-mCh expressed off the p415 plasmid were grown to an OD600 of 0.95 at 30 °C. ( D ) SRP14-GFP was overexpressed off the p413-PL plasmid and Ded1-DQAD-mCh was overexpressed off the p416-PL plasmid until an OD600 of 0.4 at 30 °C in the xpoI-T539C yeast strain. Cells in the insert were treated with 10 µg/µL (~200 nM) of leptomycin b for 1 h. The arrowheads indicate the positions where Ded1-DQAD and SRP14 colocalize within a crescent-shaped region of the nucleus.

    Journal: Molecules

    Article Title: The RNA Helicase Ded1 from Yeast Is Associated with the Signal Recognition Particle and Is Regulated by SRP21

    doi: 10.3390/molecules29122944

    Figure Lengend Snippet: Cellular location of Ded1 relative to the ER and SRP proteins. ( A ) Ded1-DQAD-GFP was expressed in the sec62 temperature-sensitive mutant with the integrated KAR2-RFP plasmid and grown to an OD600 of 1.0 at 24 °C. ( B ) The same cells as in A were incubated for 15 min at the non-permissive temperature of 37 °C prior to visualization. The arrowheads indicate positions where chains of Ded1-DQAD-GFP foci co-associated with Kar2-RFP in panels B and C. ( C ) SRP14-GFP expressed from the chromosome and Ded1-DQAD-mCh expressed off the p415 plasmid were grown to an OD600 of 0.95 at 30 °C. ( D ) SRP14-GFP was overexpressed off the p413-PL plasmid and Ded1-DQAD-mCh was overexpressed off the p416-PL plasmid until an OD600 of 0.4 at 30 °C in the xpoI-T539C yeast strain. Cells in the insert were treated with 10 µg/µL (~200 nM) of leptomycin b for 1 h. The arrowheads indicate the positions where Ded1-DQAD and SRP14 colocalize within a crescent-shaped region of the nucleus.

    Article Snippet: As a marker, we used the integrated red-fluorescent-tagged amino-terminal domain of Kar2 fused to the HDEL ER retention signal (YIPlac204TKC-DsRed-Express2-HDEL; Addgene, Watertown, MA, USA) in the two sec strains; Kar2 is an ATPase that functions as a protein chaperone for refolding proteins within the lumen of the ER ([ ] and reference therein), and consequently, the Kar2 chimera serves as a marker of the ER lumen.

    Techniques: Mutagenesis, Plasmid Preparation, Incubation